CyTOF & High-Dimensional Cytometry Core

The Georgia Cancer Center CyTOF & High-Dimensional Cytometry Core (RRID: SCR_025747) bridges the gap between the cutting-edge flow cytometry technologies and your research goals.

We provide end-to-end solutions from experimental and panel design to advanced high-dimensional data analysis.鈥�

Mass cytometry, or CyTOF (cytometry by time-of-flight), integrates the principles of flow cytometry with the quantitative capabilities of mass spectrometry, enabling multi-parametric single-cell analysis with exceptional throughput and resolution. By employing metal isotopes for antibody labeling, mass cytometry theoretically provides up to 140 detection channels with minimal signal spillover between channels.

CyTOF XTCyTOF-XT from Multiplex Bio
(formerly Standard Biotools)
has been in operation at the core since 2022.

Contact Us

Mass Cytometry

Health Sciences Campus

CN4212, CN3140

cytof@augusta.edu

The Breakthrough Single-Cell Technology

Single-Cell Technology

 


Mass Cyto vs. Fluro image

 

FLUORESCENCE FLOW CYTOMETRY
VS.		 MASS CYTOMETRY (CyTOF) 
 Broad, overlapping emission spectra  Signal Generation Discrete metal isotope masses
Compensation or spectral unmixing required Data Processing No compensation
Spillover spreading error Data Quality Minimal channel overlap
Affected by autofluorescence Background No autofluorescence
Tandem dye instability Reagent Stability Stable metal isotopes
Limited multiplexing (10-45 parameters typical) Multiplexing Capacity 50+ markers routinely 
Complex panel design and optimization Panel Design More straightforward panel design
Live-cell sorting possible  Cell Recovery Cells are destroyed during analysis
Fast acquisition (high event rate) Acquisition Speed Slower acquisition (lower event rate)

Fluorescence flow cytometry vs. Mass Cytometry (CyToF)


Mass cyto image

 

Optimized Ready-to-Go panels for human and mouse samples, easily customizable

And Many More in Core Ab bank (list of antibodies)

Your One-Stop Solution

Listing of services provided

Services Provided

 

Pricing

                    • Core Abs: $7/ul;
                    • Human MDIPA kit (30-Ab tube): $300/unit
                    • Barcoding: Pd-$50/sample
                      • CD45 or TeMal- $7/ul
                    • Ab conjugation kit: $150-$175/Rxn
  • Staff-operated CyTOF-XT usage: $100/hr
  • Technical assistance: $75/hr
  • Initial consultation, Letter of Support: FREE

Contact Us

photo of Jiji Xie, PhD

Jiji Xie, PhD

  • Director, Scientific Core
  • CN 3140

(706) 721-4157

photo of Dr. De-huang Guo

Dr. De-huang Guo

  • Research Associate
  • CN 4212

Reduce the Burden

The Georgia Cancer Center at 麻豆官网首页入口 is dedicated to reducing the burden of cancer in Georgia and across the globe through superior care, innovation, and education. Through unprecedented expansion, the Georgia Cancer Center is providing access to more first-in-the-nation clinical trials, world-renowned experts and life-saving options.

Education & Research


706-721-0570
cancer@augusta.edu


706-721-6744

 

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